MININSECT Evaluate and quantify contamination of the insect matrix using a metagenomic approach via MinION sequencing technology [PULSAR]

Context:

The expected advances in the use of metagenomics promise a significant revolution in our understanding of the diversity of the microbiome associated with the food matrix of insects. During this project, the development of tools for the descriptive and functional characterization of microbial ecosystems (MinION sequencing technology and associated bioinformatics processing) aims to evaluate the microbiome of a new food matrix studied in the unit in terms of food safety. This project will thus make it possible to accurately identify and quantify contamination by pathogenic and spoilage bacteria at key stages of insect processing, thereby enhancing product safety and quality.
In addition, this approach not only identifies known microorganisms, but also reveals new and/or understudied species that could play a major role in consumer health. Indeed, official controls or microbiological self-checks are carried out using methods that are standardized, but not necessarily adapted to this new matrix consisting of whole animals (with their digestive tracts).

 The bacterial strains present in this matrix may also differ from those identified in conventional foodstuffs. 

Aims:

Thus, the characterization and genomic comparison of strains isolated from this matrix can help optimize the development of standardized analytical methods by providing a better understanding of bacterial behavior associated with the insect matrix, including its persistence, sporulation capacity, toxin production, and response to environmental stress.
This work will inform public authorities in ensuring the safety of these new foods and will serve as scientific support for industrial sectors involved in the development of entomophagy.

Start date: june 2024

Duration: 2 years

Coordination : Boris Misery, Assistant-professor at SECALIM

Funding: Region Pays de la Loire PULSAR